Development of a Marvellous Polyherbal Formulation BASANT that Eliminates HPV-16 Preventing Carcinoma of Cervix and Various Undesirable Vaginal Infections.
G.P. Talwar 1*, Rajyashree Sharma 2, Aruna Mittal 3, Sajad A. Dar 4, Jagdish C. Gupta5,Alok C. Bharti6
1 Talwar Research Foundation, New Delhi.
2 Jawaharlal Nehru Medical College, Aligarh, Uttar Pradesh.
3 Institute of Pathology, Safdarjung Hospital Campus, New Delhi.
4 Talwar Research Foundation, New Delhi.
5 Talwar Research Foundation, New Delhi.
6 Zoology Department of Zoology, University of Delhi, New Delhi.
*Corresponding Author: G.P. Talwar, Director Research Talwar research Foundation E-8, Neb Valley, New Delhi-110068, India. Tel: 00962795420286; Fax: 00962795420286; E-mail: gptalwar@gmail.com
Citation: GP Talwar, Rajyashree Sharma, Aruna Mittal, Sajad A Dar, Jagdish C Gupta et al. (2023)Development of a Marvellous Polyherbal Formulation BASANT that Eliminates HPV-16 Preventing Carcinoma of Cervix and Various Undesirable Vaginal Infections. Cancer Prog Diagn 7: 138.
Received: March 24, 2023; Accepted: April 15, 2023; Published: April 20, 2023.
Carcinoma of cervix is a major killer of women due to Cancers. It is the second largest cancer of women worldwide, with 604,100 new cases and 341,831 deaths in 2020 [1]. In India also, it is the second leading cause of cancer deaths for females [2]. The American Cancer Society’s estimates about 13,960 new cases of invasive cervical cancer and about 4310 deaths in the United States in 2023[https://www.cancer.org/cancer/cervical-cancer/about/key-statistics.html].
Carcinoma of cervix is caused by a virus, Human Papilloma Virus (HPV). HPV-16 and/or HPV-18 infection of cervical cells renders Pap smear abnormal and leads through various stages to carcinoma of cervix. We have developed a Polyherbal formulation named BASANT (meaning Spring season), which has praise-worthy properties of not only killing HPV-16/18 but also eliminating this virus entrenched in cervical cells. Reproduced below are the results of a clinical trial concluded in 11women positive for HPV. They were administered a Capsule of BASANT intra-vaginally every night for 30 days. On re-examination, 11 out of 11women became HPV negative.
Table 1a: Pre and post treatment with BASANT of HPV-16 positive patients [3].
| S.No. |
Age |
Parity |
HPV-16 Pre-treatment |
HPV-16 Post-treatment |
| 1 |
42 |
3+0 |
+VE |
NEGATIVE |
| 2 |
27 |
4+0 |
+VE |
NEGATIVE |
| 3 |
35 |
3+0 |
+VE |
NEGATIVE |
| 4 |
28 |
1+1 |
+VE |
NEGATIVE |
| 5 |
45 |
3+0 |
+VE |
NEGATIVE |
| 6 |
35 |
4+0 |
+VE |
NEGATIVE |
| 7 |
30 |
2+1 |
+VE |
NEGATIVE |
| 8 |
45 |
2+0 |
+VE |
NEGATIVE |
| 9 |
38 |
5+2 |
+VE |
NEGATIVE |
| 10 |
35 |
3+1 |
+VE |
NEGATIVE |
| 11 |
38 |
3+1 |
+VE |
NEGATIVE |
Polyherbal formulation named as BASANT, is composed of Curcumin (diferuloyl methane), Aloe vera, purified extracts of Amla (Emblica Officinalis) and Neem (Azadirachta Indica) along with pharmacopially approved excipients [4]. It is dispensed in powder form in Capsules.
BASANT is also effective against a variety of infections such as all WHO strains of Neisseria gonorrhoeae, including those resistant to antibiotics such as Penicillin, Tetracycline, Nalidixic acid and Ciprofloxacin (Table 1).
Table 1b: WHO strains of Neisseria gonorrhoeae inhibited by BASANT [4].
|
Standard WHO strains Antibiotic resistance
|
Growth at different concentrations (v/v) of Basant after 24 h |
|
|
5% 2% 1% 0% |
||
|
None |
- - + + |
|
Tetracycline (TRNG), nalidixic acid |
- - - + |
|
Penicillin (PPNG), nalidixic acid, ciprofloxacin, ceftriaxone less sensitive |
- - - + |
|
Penicillin (PPNG), nalidixic acid, ciprofloxacin, ceftriaxone less sensitive |
- - - + |
|
Nalidixic acid, ciprofloxacin |
- - - + |
|
Nalidixic acid, ciprofloxacin |
- - + + |
|
Nalidixic acid, ciprofloxacin |
- - + + |
|
Nalidixic acid, ciprofloxacin |
- - - + |
|
Penicillin (PPNG), tetracycline (TRNG), nalidixic acid, ciprofloxacin |
- - - + |
|
Penicillin (PPNG), nalidixic acid, ciprofloxacin |
- - - + |
|
Penicillin (PPNG), tetracycline (TRNG), nalidixic acid, ciprofloxacin less sensitive |
- - + + |
BASANT has also shown efficacy against Candida glabrata, Candida albicans and Candida tropicalis including strains resistant to azole drugs and amphotericin B (Table 2). BASANT inhibits Chlamydia trachomatis, whether present in free-state or within a cell [5].
Table 2: Clinical isolates of Candida species inhibited by BASANT [4].
|
Clinical isolates of Candida Species Antibiotic resistance |
Growth at different concentrations (v/v) of Basant after 24 h |
||||
|
5% |
2% |
1% |
0% |
||
| C. glabrata ATCC 90030 |
None |
0 |
0 |
0-0.064 × 108 CFU/mL |
1.38-1.49 × 108 CFU/mL |
| C. glabrata (7 clinical isolates) |
None |
0 |
0 |
0-0.090 × 108 CFU/mL |
1.30-1.50×108 CFU/mL |
| C. glabrata (2 clinical isolates) |
Fluconazole, itraconazole, ketoconazole, voriconazole, amphotericin B |
0 |
0 |
0-0.075 × 108 CFU/mL |
1.40-1.50 × 108 CFU/mL |
| C. albicans ATCC 36082 |
None |
0 |
0 |
0-0.077 × 108 CFU/mL |
1.40-1.47 × 108 CFU/mL |
| C. albicans (5 clinical isolates) |
None |
0 |
0 |
0.05-0.08 × 108 CFU/mL | 1.37-1.50 × 108 CFU/mL |
| C. albicans |
Fluconazole, itraconazole, ketoconazole, voriconazole, amphotericin B |
0 |
0 |
0-0.067 × 108 CFU/mL |
1.33-1.40 × 108 CFU/mL |
| C. tropicalis (2 clinical isolates) |
None |
0 |
0 |
0.04-0.07 × 108 CFU/mL | 1.35-1.41 × 108 CFU/mL |
To sum up, BASANT is a marvelous formulation totally free of side effects and effective against a whole range of vaginal infections, and eliminates HPV16 from the infected cells on path of progression to carcinoma of cervix. BASANT is passed onto M/s Bipha Drugs, Kottayam, Kerala for making it available to women all over the world at a modest cost.
References
- International Agency for Research on Cancer. (2020) Globocan.
- India State-Level Disease Burden Initiative Cancer Collaborators. The burden of cancers and their variations across the states of India: The Global Burden of Disease Study 1990–2016. (2018) Lancet Oncol 19: 1289–1306.
- Talwar GP, Sharma R, Singh S, Das BC, Bharti AC et al. (2015) BASANT, a Polyherbal Safe Microbicide Eliminates HPV-16 in Women with Early Cervical Intraepithelial Lesions. Journal of Cancer Therapy 6: 1163-1166.
- Talwar GP, Dar SA, Rai MK, Reddy KV, Mitra D, et al. (2008) A novel Polyherbal microbicide with inhibitory effect on bacterial, fungal and viral genital pathogens. Int J Antimicrob Agents 32: 180–185.
- Bhengraj AR, Dar SA, Talwar GP, Mittal A et al. (2008) Potential of a novel polyherbal formulation BASANT for prevention of Chlamydia trachomatis infection. Int J Antimicrob Agents 32: 84–88.