Mohamed Shafi Bin Mahboob Ali^1*

¹General Surgeon,100, Jalan Masjid Kapitan Keling, 10200, Penang, Malaysia.

*Corresponding Author:Mohamed Shafi Bin Mahboob Ali, General Surgeon,100, Jalan Masjid Kapitan Keling, 10200, Penang, Malaysia, Tel: +601114724649; Fax: +601114724649

Citation: Mohamed Shafi Bin Mahboob Ali (2023)Correlation of IL-6 and C-Reactive Protein Levels with The Stage, Differentiation and Types of Colorectal Cancer. Medcina Intern 5: 211.

Received: October 19, 2023; Accepted: October 28, 2023; Published: November 08, 2023.

Colorectal cancer (CRC) is a type of cancer in the human large intestine. Colorectal cancer is the third and second most common cancer, respectively, in men and women worldwide (1). The number of colorectal cancer cases is expected to increase by 80% by 2035, climbing to approximately 2.4 million new colorectal cancer cases and contributing to 1.3 million deaths worldwide (2).Colorectal cancer (CRC) is the commonest cancer in Malaysian men (age-standardized incidence rate 14.8/ 100,000), the second most common cancer in Malaysian women (age-standardized incidence rate 11.1/ 100,000) (3).Unfortunately, in Malaysia CRC is detected late due to several factors. According to the research conducted in Malaysia, factors that cause delay in diagnosing CRC are lack of awareness, denial, negative perceptions of the disease, over-reliance on traditional medicine, misperceived risk, emotional barriers and negative perceptions towards screening (4, 5, and 6). Mortality due to colorectal cancer is increasing, and it is the fourth leading cause of cancer death in the world (1). The ‘rise’ of colorectal cancer in developed countries can be attributed to the increasingly ageing population, unfavorable modern dietary habits and an increase in risk factors such as smoking, low physical exercise and obesity. The development of CRC has been linked with C-reactive protein and Interleukin-6, which are systemic markers of inflammation. Several prospective studies have investigated the association of prediagnostic circulating C-reactive protein concentrations with the development of colorectal cancer, but the results have been inconsistent(7).Many research have been concentrated in studying the role of Interleukin-6,which is a type of hematopoietic-mediated inflammatory cytokine that activates lymphocytes in the initiation of colorectal cancer(8).Other factors that may promote the development of colorectal cancer such as growth factors, cytokines, and genetic or epigenetic alterations in specific oncogenes or tumor-suppressor genes, play a role during the adenoma-carcinoma sequence(9).

Research Design

Cross-sectional study.

Study Area

Department of General Surgery, Hospital University Sains Malaysia.

Protocol

JEPeM Code

USM/JEPeM/21070514

Study Population

We recruited a sample of 46 patients that were diagnosed with colorectal cancer by means of colonoscopy examination, imaging as well as clinical. The presence of colorectal cancer was confirmed with histopathological examination of rectal biopsy or colonoscopy prior to biopsy. All these patients required surgical interventions for their diseases. Out of 46 patients, 28 patients were male while the remaining 18 patients were females.

Subject Criteria

Inclusion criteria

1. Age between 18-80 years old.
2. Newly diagnosed or established diagnosis of colorectal cancer-by colonoscopic examination/imaging/histopathological.
3. Any stages of colorectal cancer (stage 0-4).

Exclusion Criteria

1. Mentally not sound to give consent.
2. Inflammatory bowel disease (IBD)
3. Damaged/defective blood samples.
4. The presence of another malignancy in the same patient-synchronous tumor.

Withdrawal Criteria

1. Change of Mind.
2. Found to be outside the parameters of inclusion criteria and inside the parameter of the exclusion criteria.
3. Breach of Protocol.

Sample size estimation

Sample size were calculated for each objective with type 1 error of 5%, Type 2 error of 20 % (80% power of study) and ratio of control to the experimental group.

For objective 1, no sample size calculation needed as descriptive analysis will be used to describe the sample only.

For objective 2, no sample size calculation needed as descriptive analysis will be used to describe the sample based on the grouping only, no hypothesis testing needed.

For objective 3, The sample size calculation was done using “Sample size calculator 2.0” by Arifiin (2017) for sample size of specific objective 3 (Pearson’s Correlation-Hypothesis Testing) based on parameter’s value from Hidayat et al. (2021). The calculation was done using all the parameters listed below:

Sample size calculation:

Using Pearson’s Correlation-Hypothesis Testing

Expected correlation(r):0.4

Significance level (α):0.05

Power (1-β):80%

Sample size=46

Expected dropout rate: 10%

Sample size with additional 10% dropout: 52

Hidayat F, Labeda I, Sampetoding S, Pattelongi IJ, Lusikooy RE, Warsinggih, Dani MI, Mappincara, Kusuma MI, Uwuratuw JA, Syarifuddin E, Faruk M. Correlation of interleukin-6 and C-reactive protein levels in plasma with the stage and differentiation of colorectal cancer: A cross-sectional study in East Indonesia. Ann Med Surg (Lond). 2021 Jan 23; 62:334-340. doi: 10.1016/j.amsu.2021.01.013. PMID: 33552492; PMCID: PMC7847822.

For objective 4, The sample size calculation was done using “Sample size calculator 2.0” by Arifiin (2017) for sample size of specific objective 4 (two mean formula) based on parameter’s value from Hidayat et al. (2021).

The largest sample size was taken as the final sample size and after anticipating 10% dropout rate, the corrected sample size was 46 patients.

Subject Recruitment

Subject recruitment-All patients that were previously diagnosed and was newly diagnosed with colorectal cancer by the means of colonoscopy, imaging and clinical, who fulfills the inclusion and the exclusion criteria’s by the Department of General Surgery in Hospital Universiti Sains Malaysia (HUSM).

Subjects for the study was recruited from the following pool:

Randomization and Masking

No randomization or masking were applied in this study as this is a cross sectional study involving 46 patients who were confirmed to have colorectal cancer.

- Manufactured by BIOLEGEND WAY SAN DIEGO

-expiry date is within 2 years of purchase

-sensitivity: 0.8pg/ml

-range: 1.56-50pg/ml

-Catalogue No: 430507

-Manufactured by Aidian Oy Koivu-Mankkaan tie 6 B, FI-02200 Espoo, Finland

-Reproducible CRP results within a range of 5 - 200 mg/l

-Results within 2 minutes

-Lyophilized reagents containing <1% of sodium azide

-Catalogue No: 153763

-The centrifuge machine that was used for the study

-Subject’s blood sample was centrifuged in the machine at 4500RPM for 5minutes to separate the serum from the red blood cells.

-The spun serum was kept in the freezer under temperature between -2 degree to -28 degree Celsius.

-Analyze and interpret patient’s serum C-reactive protein levels at the speed of 60T/Hour

-Detection using laser device and integrated detection system.

-The instrument carries out detections after it is conjugated with ELISA reagent.

-First, it detects absorbencies of under detection sample and standard liquid.

-Secondly, it performs analyses and calculations.

-Finally, it produces values or conditions of the under-detection material in the sample liquid.

-The ELISA technique used was capture assay ‘sandwich’ method.

-Optical density is used to detect the level of the primary antibodies bound to the proteins at the bottom of the wells.

-ELISA results can be displayed into three types: quantitative, qualitative and semi-quantitative.

Data Collection Method

This cross-sectional study, conducted at the Hospital University Sains Malaysia (HUSM), followed a protocol approved by the JEPeM USM, which is an ethical body of the university with the registration code:USM/JEPeM/21070514,dated 20th December 2021 in accordance with the criteria of strengthening the reporting of cohort studies in surgeries(10). A total of 46 patients who were diagnosed with colorectal cancer were recruited into the studies (28 males and 18 females) aged 56.11 years on average with mean value of 15.51. All these subjects needed surgical treatment due to CRC, the presence of which was confirmed by histopathological examination with a rectal biopsy or colonoscopy prior to biopsy. To be eligible to participate, patients had to have a diagnosis of CRC based on a histopathological examination, demonstrate normal liver function, kidney function, urinalysis and chest x-rays, have no history of hemostatic disorders, not currently suffering from acute or chronic infectious diseases as evidenced by routine blood results and consent to participate. Exclusion criteria were damaged blood samples or malignancy in another organs (i.e., synchronous tumor). Stages of CRC were determined with reference to the American Joint Committee on cancer’s 2017 TNM staging system (11), whereas grades of differentiation were determined based on the grading of the World Health Organization (12,13).

Sample Examination

Before the subjects underwent colorectal surgeries, they were counselled by the principal investigator regarding the details of the studies. Once the subject understands the study details, consent will be taken from them. After obtaining written consent, about 3ml to 5ml venous blood will be withdrawn from the subject. Immediately the blood will be transferred to a yellow plain tube, label with the subject’s unique ID and put in a biohazard bag. The principal investigator will then run the sample in a centrifuge machine for about 4500rpm for 5minutes.The obtained serum will be separated into two aliquots. One aliquot was stored at 080 degree for the later analysis of IL-6 serum concentration, namely with an enzyme linked immunosorbent assay (ELISA) kit (Legend Max Human) while another aliquot was tested for C-reactive protein serum level using CRP kit (Quick Read Go CRP).Once 46 samples obtained, the principal investigator run the test for both IL-6 and CRP on the same day with the help of HUSM lab assistant.IL-6 level were processed using sandwich ELISA method with the Legend Max Human kit according to the manufacturer’s instructions.(14-16).C-reactive protein was processed using Quick Read Go kit according to the manufacturer’s instructions. Measurement of IL-6 concentration was expressed in pictograms per milliliter (pg/ml) while CRP concentration was expressed in milligrams per liter (mg/L).

Data was entered and analyzed using IBM SPSS (Statistical Package for the Social Sciences) version 26. Data were screened for missing values prior to the analysis. From the total of 50 samples, a total of 46 usable sets of observations were used for subsequent analyses. Then, descriptive statistics to describe the samples used in the present study were used. All categorical variables were summarized as frequency (n) and percentage (%), while numerical variables were presented as mean and standard deviation (SD). Then, subsequent analysis of descriptive statistic was used to demonstrate the difference mean Serum IL-6 between different stages of TnM. Then, Pearson correlation test was used to examine the relationship between mean levels of Serum IL-6 with mean CRP. The analysis was continued with summarizing the upregulation of Serum IL-6 in CRC in HUSM population between different aspects (stages, types of CRC and level of differentiation) using Kruskal-Walli’s test with post-hoc comparison of Mann-Whitney test. It was to assess the difference in median levels of Serum IL-6 CRP at each stage, types and differentiation level of CRC. The same analysis was used for examining the association between stages, types of CRC and differentiation level towards CRP level. The graphical illustration of bar chart, pie chart and box- plot were used to explain the distribution of all related variables individually or by combination for all the samples used in the present study. The scatter plot was used to show the relationship between the mean serum level of CRP and Serum IL-6. All values less than 0.05 will be considered statistically significant.

Participants Characteristic

A total of 46 samples were included for this study. The data obtained was expressed as mean with standard deviation (SD) for numerical variables and frequency (n) with percentage (%) for categorical variables as tabulated in Table 1.

Table 1: Descriptive statistics for all patient’s characteristics.

Comparison of serum IL-6 by stages.

Based on Table 2, the mean serum IL-6 was different between different stages of the cancer. In general, the highest mean of serum IL-6 was found in stage I and the mean reduced with increase in level of stages.

Table 2: Comparison serum IL-6 value between different stages of TnM.

Correlation of IL-6 and CRP

There was a significant positive moderate correlation found between IL-6 and the mean value of CRP. 

Table 3: Correlation between related variables.

Comparison of mean value CRP and serum IL-6 between different groups of variables.

Table 4 and 5 show the comparison of CRP and serum Il-6 between different stages, types of CRC and level of differentiation.

Based on Table 4, there were no significant association between related variables towards CRP value except for level of differentiation (p=0.026). The highest median value of CRP was found in the well differentiation group (median=96.00, IQR=89.00).

Table 4: Comparison of mean values of CRP between different groups of related variables.

* Kruskal Wallis test was applied. No IQR value for some variables because of only 1 case or constant found in that group.

Based on Table 5, there were no significant association between related variables towards serum IL-6 value for stages, types of CRC and level of differentiation (all p>0.050).

Table 5: Comparison of serum IL-6 between different groups of related variables.

* Kruskal Wallis test was applied. No IQR value for some variables because of only 1 case or constant found in that group.

Graphical Illustration of all related variables

All of the charts below illustrated and summarized the distributions of all related variables by each variable individually and in combination.

Figure 1: Scatter plot of association between Serum IL-6 levels with mean CRP.

Figure 2: Distribution of stages of TnM amongst the total sample.

Figure 3: Distribution of different types of cancer amongst the total sample.

Figure 4: Distribution of cancer differentiation amongst the total sample.

Figure 5: Distribution of diet status amongst the total sample.

Figure 6: Distribution of ethnicity amongst the total sample.

Figure 6b: Distribution of smoking status amongst the total sample.

Figure 7: Distribution of Serum IL-6 between different stages amongst the total sample.

Figure 8: Distribution of gender status amongst the total sample.

Figure 9: Distribution of CRP level between different differentiation levels among the total samples.

Figure 10: Distribution of Serum IL-6 level between different differentiation levels among the total samples.

Figure 11: Distribution of CRP level between different cancer types among the total samples.

Figure 12: Distribution of Serum IL-6 level between different cancer types among the total samples.

Figure 13: Distribution of CRP level between different cancer stages among the total samples.

Figure 14: Distribution of Serum IL-6 level between different cancer stages among the total samples.

We recruited 46 patients that were diagnosed with colorectal cancer. Out of 46 patients, 28 or 60.87% were males while 18 or 39.13% were females. We faced recruitment limitations as the study was conducted during the COVID-19 pandemic era where most of the hospital resources and departments were concentrated in treating and managing COVID-19 patients. Thus, the level of screening and detections of colorectal cancer drastically fell. The mean age of all 46 subjects were observed as 56.11 years old with standard deviation of 15.51. This value is higher compared to the studies done by Hidayat et al (16) where the mean age was 50 years old. Male sex manifested the most for the development of colorectal cancer while ethnic wise, we observed that ethnic Malays has the highest numbers, followed by Chinese while no Indian ethnic was diagnosed with colorectal cancer in our centre. This finding corroborates with K-L Goh’s (18) data which gave us insight into Malaysia’s colorectal demographic trends. This trend is most probably due to the preponderance of the cancer to the elderly male while ethnic wise, Malay’s ethnic has the highest number due to the population factor that is the study was conducted in Kelantan, which is the East-Coast region of Malaysia with Malays majority. While different data was observed in Penang, a small island at the Northern region of Malaysia’s peninsular where the Chinese ethnic recorded the greatest number of cases in colorectal cancer (19). We also observed high percentages in those colorectal cancer patients that are smokers and non-vegan. These findings correlated with the findings of Nuri Faruk (20) that processed red meat significantly increases the development of colorectal cancer by about 20-30%.

The mean CEA recorded in our subject was 214.04ng/ml with the standard deviation of 860.50, while the commonest region of tumor location was in colon compared to the rectum. The distribution of cancer stages was according the cancer stages where stage 4 recorded the most, followed by stage 3,2 and lastly stage 1.

Interleukin-6, which is a pro-inflammatory cytokine in the development of colorectal cancer, was studied their levels in different stages of colorectal cancers (21).It was found that colorectal cancer stage 2 recorded the highest level of IL-6, which was 326.67pg/ml with the standard deviation of 219.69,followed by colorectal cancer stage 1 with the value of 259.93pg/ml(S.D 370.66),stage 4 with the value of 109.90pg/ml(S.D 160.95) and lastly, stage 3 with the Interleukin level of 79.49pg/ml(157.89).These findings were contraindicated with the study conducted by Heike Knüpfer which stated that higher IL-6 levels are associated with increasing tumor stages(22).Subjects used in Heike Knüpfer was far smaller compared to ours and that may influenced the outcome.

We found a significant positive moderate correlation between IL-6 and mean value of CRP with a Pearson correlation value of 0.603 and p value of

We tested both CRP and IL-6 with sandwich ELISA test and derived a scatter plot graph. The findings of the graph was a non-linear curve (Figure 1). CRP and IL-6 data for all 46 subjects were randomly scattered both on the upper and lower area of the plot. This shows that CRP and IL-6 have a non-linear relationship in the development of colorectal cancer. Our findings was totally different compared to the findings by our Indonesian colleagues F.Hidayat et al (17) in which they obtained a linear relationship between CRP and IL-6 in colorectal cancer patients. This can be due to many factors, such as the kit used, the lab technician that performed the test as well as the reagent used. A retrospective review of our data might show a different result.

Figure 2 shows the graphical distribution of the colorectal cancer stages in which stage 4 has the most numbers, 30 subjects (65.22%), followed by stage 3, 2 and lastly stage 1. While figure 3 shows us the different types of cancer found in our subjects which mostly were adenocarcinoma, 38 subjects (82.61%), followed by other types of cancer such as signet cell cancer. We had 2 subjects with hamartoma while 1 subject each for lymphoma and GIST. These findings were consistent with the cases that were commonly found in our center which is Adenocarcinoma of the colon. Although other types of colon cancers have been reported, Adenocarcinoma takes the precedence. Figure 4 shows the distribution of the types of differentiation of the cancer in which moderately differentiated colorectal cancer was the highest with 22 subjects (47.83%) followed by well differentiated and lastly was the poorly differentiated. This distribution indicates that most of our patients have a good to moderate prognosis.

Figure 7 depicting the levels of IL-6 amongst different stages of colorectal cancer in our study. We found that stage 4 has the highest IL-6 levels followed by stage 3, 2 and lastly stage 1. These findings corroborated with the studies done by Farideh Esfandi et al in which they concluded that the levels of IL-6 will be higher with the advancement of the cancer stages (24).

The box plot in Figure 9 shows that plasma CRP in various colorectal cancer differentiation were not normally distributed and the mean level was highest in the well differentiated group. While box plot in Figure 11 also shows that the level of CRP and the type of cancers were not normally distributed with other types of cancer documented the highest mean value. Box plot in Figure 14 shows that CRP levels in different stages of cancer were not normally distributed either with stage 2 recorded that highest mean. Thus, there are no significant correlations between the CRP value and the CRC stages, types or differentiation. Similar findings were detected in Figure 10, 12 and 14 box plot for the IL-6 where there were no significant correlations between the IL-6 values and the stages, differentiation and the types of the CRC. These findings were consistent with a meta-analysis done by Bo Zhou et al where they found that there was no direct correlation between CRP and IL-6 with the CRC development (25).

The study is confined to a single center with a limitation of the varieties of subject. The majority of our subjects were Malays as the study was conducted in HUSM Kubang Kerian,Kelantan where the majority population were Malays. Other races such as Chinese and Indians were very scarce making the studies racial distribution uneven. Our sample size was also small, only 46 subjects as the studies were conducted during the COVID-19 pandemic. Many patients were turned back as the infection rates and the number of cases surged. Another limiting factor was the duration of the studies was quite short. A better outcome might be produced if the study could be extended. Some of the samples were unevenly distributed due to the small number of subjects and other factors.

Our study demonstrated that there is a significant positive moderate correlation between IL-6 and mean value of CRP with Pearson correlation value of 0.603 and a p-value of

1. J Ferlay, I Soerjomataram, R Dikshit, et al. (2015) Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer 136 E359-E386.
2. Douaiher J, Ravipati A, Grams B, Chowdhury S, Alatise O, et al. (2017) Colorectal cancer-global burden, trends, and geographical variations. J. Surg. Oncol 115: 619–630.
3. Ministry of Health Malaysia (2020) Malaysia National Cancer Registry report 2012-2016. Putrajaya: National Cancer Institute.
4. Khan TM, Leong JPY, Ming LC, Khan AH (2015) Association of knowledge and cultural perceptions of Malaysian women with delay in diagnosis and treatment of breast cancer: a systematic review. Asian Pac J Cancer Prev 16:5349–5357.
5. Yu FQ, Murugiah MK, Khan AH, Mehmood T (2015) Meta-synthesis exploring barriers to health seeking behaviour among Malaysian breast cancer patients. Asian Pac J Cancer Prev 16: 145–152.
6. Azeem E, Gillani SW, Poh V, Sulaiman SAS, Baig MR et al. (2016) Barriers to colorectal cancer screening in Asia: a systematic review. Trop J Pharm Res 15: 1543–1548.
7. Tsilidis KK, Branchini C, Guallar E, Helzlsouer KJ, Erlinger TP, et al. (2008) C-reactive protein and colorectal cancer risk: a systematic review of prospective studies. Int J Cancer 123: 1133-1140.
8. Becker C, Fantini MC, Wirtz S, Nikolaev A, Lehr HA, et al. (2005) IL-6 signaling promotes tumor growth in colorectal cancer. Cell Cycle 4: 217- 220. PMID: 15655344
9. Turano M, Cammarota F, Duraturo F, Izzo P, De Rosa M, et al. (2021) A Potential Role of IL-6/IL-6R in the Development and Management of Colon Cancer. Membranes (Basel) 11: 312. PMID: 33923292; PMCID: PMC8145725
10. Agha R, Abdall-Razak A, Crossley E, Dowlut N, Iosifidis C, et al. STROCSS (2019) Guideline: Strengthening the reporting of cohort studies in surgery. Int J Surg 72: 156-165. PMID: 31704426
11. Amin MB, Greene FL, Edge SB, Compton CC, Gershenwald JE, et al. (2017) The Eighth Edition AJCC Cancer Staging Manual: Continuing to build a bridge from a population-based to a more "personalized" approach to cancer staging. CA Cancer J Clin 67: 93-99. PMID: 28094848
12. Ariani, G D W, Faruk, M. (2020). Colorectal neuroendocrine tumors: A case series. International Journal of Surgery Case Reports 72: 411-417.
13. Nagtegaal ID, Odze RD, Klimstra D, Paradis V, Rugge M, et al. (2019) WHO Classification of Tumours Editorial Board. The 2019 WHO classification of tumours of the digestive system. Histopathology 76: 182-188. PMID: 31433515; PMCID: PMC7003895
14. Oley MH, Oley MC, Langi FLFG, Langi YA, Keppel BJ, et al. (2021) Predicting hyperbaric oxygen therapy success using the decision tree approach. Ann Med Surg (Lond) 69: 102725. PMID: 34457258; PMCID: PMC8377532
15. Warsinggih Liliyanto Prihantono, Ariani GDW, Faruk M. (2020) Colorectal neuroendocrine tumors: A case series. Int J Surg Case Rep 72: 411-417. PMID: 32563832; PMCID: PMC7306531
16. Sachraswaty, R Laiddinga, Fonny Josha, M.Faruk, Warsinggihb Agussalim Bukharid Muh Nassrum Massie, et al. (2020) The effect of combined platelet-rich plasma and stromal vascular fraction compared with platelet-rich plasma, stromal vascular fraction, and vaseline alone on healing of deep dermal burn wound injuries in the Wistar rat,Ann. Med Surg 60: 737-742.
17. Hidayat F, Labeda I, Sampetoding S, Pattelongi IJ, Lusikooy RE, et al. (2021) Correlation of interleukin-6 and C-reactive protein levels in plasma with the stage and differentiation of colorectal cancer: A cross-sectional study in East Indonesia. Ann Med Surg (Lond) 62: 334-340.PMID: 33552492; PMCID: PMC7847822
18. Goh KL, Quek KF, Yeo GT, Hilmi IN, Lee CK, et al. (2005) Colorectal cancer in Asians: a demographic and anatomic survey in Malaysian patients undergoing colonoscopy. Aliment Pharmacol Ther 22: 859-64. PMID: 16225496
19. Narimah Samat, Aishah Knight Abd Shattar, Yasmin Sulaiman, Azizah Ab Manan, Chan Ngai Weng et al. (2015) Investigating Geographic Distribution of Colorectal Cancer Cases: An Example from Penang State, Malaysia.,School of Humanities, Universiti Sains Malaysia, Penang, Accepted on September 11, 2013,Published on November 29, 2013 doi:10.5539/ass.v9n17p38   
20. Aykan NF. (2015) Red Meat and Colorectal Cancer. Oncol Rev 9: 288. PMID: 26779313; PMCID: PMC4698595
21. Waldner MJ, Foersch S, Neurath MF. (2012) Interleukin-6--a key regulator of colorectal cancer development. Int J Biol Sci 8: 1248-1253. PMID: 23136553; PMCID: PMC3491448
22. Knüpfer H, Preiss R. (2010) Serum interleukin-6 levels in colorectal cancer patients--a summary of published results. Int J Colorectal Dis 25:135-140. PMID: 19898853
23. Nikiteas NI, Tzanakis N, Gazouli M, Rallis G, Daniilidis K, et al. (2005) Serum IL-6, TNFalpha and CRP levels in Greek colorectal cancer patients: prognostic implications. World J Gastroenterol 11: 1639-1643. PMID: 15786541; PMCID: PMC4305945
24. Esfandi F, Mohammadzadeh Ghobadloo S, Basati G. (2006) Interleukin-6 level in patients with colorectal cancer. Cancer Lett 244: 76-78. PMID: 16442710
25. Zhou B, Shu B, Yang J, Liu J, Xi T, et al. (2014) C-reactive protein, interleukin-6 and the risk of colorectal cancer: a meta-analysis. Cancer Causes Control 25: 1397-1405. PMID: 25053407.